Single cell oil production by Lipomyces starkeyi: Biphasic fed-batch fermentation strategy providing glucose for growth and xylose for oil production

Effect of feeding strategies on lipid production by Lipomyces starkeyi.

The aim of this study was to produce microbial oil from Lipomyces starkeyi DSM 70296 grown in hemicellulose hydrolysate (H-H). Glucose and xylose were used for batch, fed-batch, repeated fed-batch, and continuous cultures, and H-H was tested at continuous culture. The highest cell and lipid concentrations of 85.4 and 41.8g/L, respectively, were obtained using repeated fed-batch strategy. Contin...

Evaluation of Heat Induction Strategy for Recombinant Human Growth Hormone Expression in Fed-Batch Fermentation

A modular simulation package for fed-batch fermentation: penicillin production

Simulation software based on a detailed unstructured model for penicillin production in a fed-batch fermentor has been developed. The model extends the mechanistic model of Bajpai and Reuss by adding input variables such as pH, temperature, aeration rate, agitation power, and feed flow rate of substrate and introducing the CO2 evolution term. The simulation package was then used for monitoring ...

Single Cell Oil Producing Yeasts Lipomyces starkeyi and Rhodosporidium toruloides: Selection of Extraction Strategies and Biodiesel Property Prediction

Single cell oils (SCOs) are considered potential raw material for the production of biodiesel. Rhodosporidium sp. and Lipomyces sp. are good candidates for SCO production. Lipid extractability differs according to yeast species and literature on the most suitable method for each oleaginous yeast species is scarce. This work aimed to investigate the efficiency of the most cited strategies for ex...

Fatty alcohol production in Lipomyces starkeyi and Yarrowia lipolytica

BACKGROUND Current biological pathways to produce biofuel intermediates amenable to separations and catalytic upgrading to hydrocarbon fuels are not cost effective. Previously, oleaginous yeasts have been investigated primarily for lipid production. However, yeasts store neutral lipids intracellularly making recovery difficult and expensive. In addition, once recovered from the cells, lipids ar...